本實(shí)驗(yàn)采用雙抗體夾心ELISA法。抗小鼠IgE抗體包被于酶標(biāo)板上，加入標(biāo)準(zhǔn)品和待測(cè)標(biāo)本，標(biāo)準(zhǔn)品和標(biāo)本中的IgE會(huì)與包被于酶標(biāo)板上的抗體結(jié)合，形成免疫復(fù)合物，游離的成分被洗去；加入生物素化的抗小鼠IgE抗體和辣根過氧化物酶標(biāo)記的親合素，抗小鼠IgE抗體與被捕獲的標(biāo)準(zhǔn)品及待測(cè)標(biāo)本結(jié)合，生物素與親和素特異性結(jié)合，游離的成分被洗去。加入顯色底物（顯色劑），若反應(yīng)孔中有IgE，辣根過氧化物酶會(huì)使無色的顯色劑變藍(lán)，加終止液變黃。在450nm處測(cè)OD值，IgE濃度與OD₄₅₀值之間呈正相關(guān)，可通過繪制標(biāo)準(zhǔn)曲線求出標(biāo)本中IgE濃度。

產(chǎn)品性能

1. 靈敏度：最小可測(cè)小鼠IgE達(dá)2ng/ml。

2.重復(fù)性：板內(nèi)、板間變異系數(shù)均<10%。

產(chǎn)品組分及儲(chǔ)存條件

試驗(yàn)所需自備儀器和試劑:

1. 酶標(biāo)儀(450nm檢測(cè)波長(zhǎng)濾光片，570nm或630nm校正波長(zhǎng)濾光片)。

2. 高精度加液器及一次性吸頭：0.5-10μl，2-20μl，20-200μl，200-1000μl。

3. 37℃恒溫箱、蒸餾水或去離子水、坐標(biāo)紙。

• MOUSE IL-5 ELISA KIT（EM019-96）
• MOUSE Insulin ELISA KIT（EM017-96）
• MOUSE IL-13 ELISA KIT（EM016-96）

• ELISA kit 產(chǎn)品手冊(cè)2019click to download

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