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ResiQuant? Ready-to-use CHO DNA Residue Detection Kit(CRH00-1091)

ResiQuant? Ready-to-use CHO DNA Residue Detection Kit(CRH00-1091)

ResiQuant? Ready-to-use CHO DNA Residue Detection Kit

Catalog No.
Size
CRH00-1091
50T
CRH00-1092
100T

Overview performance Related application image Data and files literature Q&A COA

       This kit uses the Taqman probe quantitative PCR method to quickly detect residual Chinese Hamster Ovary (CHO) host cell DNA in intermediates, semi-finished products and finished products of various biological products and pharmaceuticals. The kit introduces an uracil-N-glycosylase (UNG) system, which can effectively remove residual contamination of PCR products and great reduce false positives caused by contamination of amplified products. The reagent components contain internal standard control (IC), we can monitor whether the reaction process is proceeding normally and eliminate sample interference; ROX is suitable for ABI fluorescence quantitative PCR instruments or other similar equipment, and plays a role in optical path correction.

This product is suitable for biological drug solutions, finished products, semi-finished products, intermediate products or process monitoring samples produced using Chinese Hamster Ovary cells (CHO-K1,CHO-S,CHO-DG44,CHO-GS and other cell lines) as hosts. To obtain a calibration curve for quantitative sample DNA, the kit contains DNA calibrators with 6 concentration gradients (300 pg/μL to 3 fg/μL) for preparation of the calibration curve.

For residual DNA extraction from CHO host cells, it is recommended to use our company’s universal residual host DNA sample pretreatment kit. Before first use, it is recommended to complete a product suitability study: confirm whether there are interferences or inhibitors in the sample or sample matrix, and select appropriate sample detection dilution conditions.

 

Product Features

1. Quantitative Range: 3fg/μL to 300 pg/μL

2. Accuracy: DNA calibrators have a complete traceability chain, traceable to national standards;

3. Precision: high intra-batch repeatability and small inter-batch variation within the quantitative limit;

4. Exclusive: Specific detection of residual DNA in CHO host cells without interference from 293,Vero, E.coli and other foreign cells

5. Simplicity: Ready-to-use calibrators eliminate the need for cumbersome dilution operations, simplifying the experimental process; avoiding artificial differences in standard curve dilution operations, and achieving good consistency of results. 

6. Anti-interference: UNG anti-pollution system effectively removes PCR product contamination;

Internal ControlICto improve detection control of sample interference and reaction preparation

 

Product Advantages

Strong Anti-interference

UNG Anti-pollution System and Digestion ProcedureImprove the kit’s tolerance to aerosol contamination of PCR products; 

Internal Control (IC)Improve detection control of sample interference and reaction preparation;

Ready-to-use calibrator

No need for cumbersome dilution operations, simplifying the experimental process; avoid artificial differences in the standard dilution operation, and the results are consistent.

Complete kit traceability system

The DNA calibrator has a complete traceability chain, the kit traceability system is complete, the characteristics are traceable to CHO-K1(ATCC CCL-61), the value is traceable to the national standard material (270026) and the national standard material.

Supply chain security

Large batch output, short delivery time, stable supply;