本實驗采用雙抗體夾心ELISA法?？剐∈驣gE抗體包被于酶標板上，加入標準品和待測標本，標準品和標本中的IgE會與包被于酶標板上的抗體結合，形成免疫復合物，游離的成分被洗去；加入生物素化的抗小鼠IgE抗體和辣根過氧化物酶標記的親合素，抗小鼠IgE抗體與被捕獲的標準品及待測標本結合，生物素與親和素特異性結合，游離的成分被洗去。加入顯色底物（顯色劑），若反應孔中有IgE，辣根過氧化物酶會使無色的顯色劑變藍，加終止液變黃。在450nm處測OD值，IgE濃度與OD₄₅₀值之間呈正相關，可通過繪制標準曲線求出標本中IgE濃度。

產品性能

1. 靈敏度：最小可測小鼠IgE達2ng/ml。

2.重復性：板內、板間變異系數(shù)均<10%。

產品組分及儲存條件

試驗所需自備儀器和試劑:

1. 酶標儀(450nm檢測波長濾光片，570nm或630nm校正波長濾光片)。

2. 高精度加液器及一次性吸頭：0.5-10μl，2-20μl，20-200μl，200-1000μl。

3. 37℃恒溫箱、蒸餾水或去離子水、坐標紙。

• MOUSE IL-5 ELISA KIT（EM019-96）
• MOUSE Insulin ELISA KIT（EM017-96）
• MOUSE IL-13 ELISA KIT（EM016-96）

• ELISA kit 產品手冊2019點擊下載

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