產(chǎn)品概述

白介素 2（IL-2）是通過結(jié)合多分子細胞受體復合物發(fā)揮生物活力的。多年來，這個受體復合物被認為是由IL-2Rα與IL-2β兩個糖蛋白鏈組成的，兩個亞基相互結(jié)合在一起形成一個高親和力受體來傳遞 IL-2信號的。IL-2Rα（也稱為 Tac 抗原或 CD25），是一個分子量為55kD跨膜糖蛋白，有351個氨基酸組成。IL-2β在1989 年被克隆出來，它是個由575個氨基酸組成的跨膜糖蛋白，分子量為75kD，其中有286個氨基酸位于胞漿內(nèi)，更顯著地參與了細胞信號傳導作用。常認為它是IL-2一種弱抑制劑。在任何情況下，體液中增高的可溶性IL-2Rα常伴隨這T細胞與B細胞的增加與免疫系統(tǒng)的激活。

產(chǎn)品原理

本實驗采用雙抗體夾心ELISA法?？谷薎L-2 sRα 單抗包被于酶標板上，標本和標準品中的IL-2 sRα 會與單抗結(jié)合形成免疫復合物，游離的成分被洗去；加入生物素化的抗人IL-2 sRα 抗體，生物素化抗人IL-2 sRα 抗體與酶標板上結(jié)合的標準品或樣本中的IL-2 sRα 結(jié)合而形成免疫復合物，游離的成分被洗去；加入辣根過氧化物酶標記的親合素，親合素與生物素特異性結(jié)合，游離的成分被洗去。加入顯色底物（顯色劑），若反應孔中有IL-2 sRα，辣根過氧化物酶會使無色的顯色劑變藍，加終止液變黃。在450nm處測OD值，IL-2 sRα 濃度與OD₄₅₀值之間呈正相關(guān)，可通過繪制標準曲線求出標本中IL-2 sRα 濃度。

產(chǎn)品性能

1. 靈敏度：最小可測人IL-2 sRα達15pg/ml 。

2. 重復性：板內(nèi)、板間變異系數(shù)均<10%。

產(chǎn)品組分及儲存條件

試驗所需自備儀器和試劑:

1. 酶標儀(450nm檢測波長濾光片，570nm或630nm校正波長濾光片)。

2. 高精度加液器及一次性吸頭：0.5-10μl，2-20μl，20-200μl，200-1000μl。

3. 37℃恒溫箱、蒸餾水或去離子水、坐標紙。

• HUMAN Insulin ELISA KIT（EH046-96）
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• ELISA kit 產(chǎn)品手冊2019點擊下載

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Q:ResiQuant? Mycoplasma Detection Kit （CRB00-1011）

A:ResiQuant? Mycoplasma Detection Kit (Fluorescent Probe qPCR Method) is used in conjunction with ResiQuant? Mycoplasma DNA Extraction And Purification Kit (Magnetic Bead Method)（CAT. CRB00-0031/CRB00-0032）, it can qualitatively detect whether there is mycoplasma contamination in the main cell bank, working cell bank, virus seed batch and cell products. This kit uses fluorescent probe qPCR technology to design primes probes in the conserved gene fragments of mycoplasma. Based on the sequence comparison results, it can quantitatively detect about 174 species of mycoplasma. The detection is highly specific, and bacteria closely related to mycoplasma (Lactobacillus, Clostridium, Streptococcus, etc.) and common engineering cells such as CHO, VERO, HEK293, etc. are not detected. Verified according to the relevant requirements of EP 2.6.7, JP XVIII and USP <77> for mycoplasma detection, the detection limit can reach 10 CFU/mL，which meets the detection sensitivity requirements of EP 2.6.7 for the NAT method instead of the culture method. This kit introduces an uracil-N-glycosylase (UNG) anti-contamination system, which can effectively remove residual contamination of PCR products and avoid false positives caused by contamination of amplified products. This kit uses the FAM+HEX/VIC dual-channel detection method and include PC (positive control) and RC (recovery control), which are used to monitor detection sensitively and sample recovery to avoid false negatives. The reagent component contains the reference dye ROX, which is suitable for ABI fluorescence quantitative PCR instruments or other similar equipment, and plays the role of fluorescence reference light path correction. For first-time use, it is recommended to conduct a sample suitability study, including matrix interference, detection limit and specificity. The mycoplasma nucleic acid content in low-level contamination samples（≤10 CFU/mL）does not conform to the normal distribution. This kit uses a 3-well reporting method with a confidence level of more than 90%. Product Features 1.High Sensitivity: The detection limit is 10CFU/mL, compared with the culture method, the sensitivity is equivalent to or better than the culture method; 2.Wide Coverage: Approximately 174 species of mycoplasma can be detected; 3.False Positive Control: Use touch down qPCR technology to reduce non-specific amplification and background dryness, and introduce the UNG anti-pollution system to reduce contamination caused by amplification products; effectively avoiding false positives; 4.False Negative Control: Use a dual-channel detection method, including PC (positive control) and RC (recovery control), to monitor sample recovery and matrix interference to avoid false negatives; 5.Product Compliance: Verified in accordance with European Pharmacopoeia EP 2.6.7 and Japanese Pharmacopoeia JP G3, and meets regulatory requirements;

Q:ResiQuant? Mycoplasma DNA Extraction and Purification Kit （CRB00-0031）

A:Mycoplasma is a common contaminant in mammalian cell culture processes, mycoplasma contaminant can cause changes in host cell DNA, RNA and protein expression, such as interruption of DNA and RNA synthesis and changes in membrane antigens, thus affecting cell growth and proliferation. ResiQuant? Mycoplasma DNA Extraction and Purification Kit (Magnetic Bead Method) effectively reduces the interference of cells and sample matrix through pretreatment and specific binding of magnetic beads, and can extract and purify trace amounts of mycoplasma DNA in biological samples. The extraction step is simple and only takes 2~3 hours. Used in conjunction with the ResiQuant? Mycoplasma DNA Detection Kit (Fluorescent Probe qPCR Method)（CAT. CRB00-1011/CRB00-1012）, it can achieve the purpose of rapid detection of mycoplasma.