產(chǎn)品概述
ADAMTS1是與IL-12 相關(guān)的異源二聚體細(xì)胞因子,由兩個(gè)二硫鍵連接的亞基組成,一個(gè)是ADAMTS1 獨(dú)特的p19亞基,一個(gè)是與IL-12相同的p40 亞基。p19亞基具有IL-12的p35亞基的同源性,與其他單鏈的細(xì)胞因子如IL-6和IL-11也有同源性。人的p19亞基的cDNA編碼189個(gè)氨基酸殘基的前體蛋白,推斷有19個(gè)氨基酸的信號(hào)肽和170個(gè)氨基酸的成熟蛋白。人和人的p19亞基具有70%的序列同源性。功能性ADAMTS1受體復(fù)合物由兩個(gè)受體亞基組成,IL-12 Rβ 1和ADAMTS1 R亞基。
產(chǎn)品原理
本實(shí)驗(yàn)采用雙抗體夾心ELISA法。抗人ADAMTS1單抗包被于酶標(biāo)板上, 同時(shí)加入標(biāo)本、標(biāo)準(zhǔn)品和生物素化的抗人ADAMTS1抗體,標(biāo)本、標(biāo)準(zhǔn)品中的ADAMTS1會(huì)與加入于孔中的生物素化的抗人ADAMTS1抗體及包被于酶標(biāo)板上的單抗結(jié)合, 形成免疫復(fù)合物,游離的成分被洗去;加入辣根過氧化物酶標(biāo)記的親合素,親合素與生物素特異性結(jié)合,游離的成分被洗去。加入顯色底物(顯色劑),若反應(yīng)孔中有ADAMTS1,辣根過氧化物酶會(huì)使無色的顯色劑變藍(lán),加終止液變黃。在450nm處測(cè)OD值, ADAMTS1濃度與OD450值之間呈正相關(guān),可通過繪制標(biāo)準(zhǔn)曲線求出標(biāo)本中ADAMTS1濃度。
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